ZESZYT Nr 1/ 2009 Abstrakty Volume No. 1/2009 Abstracts |
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| 10.
Resistance to black root rot (Chalara elegans Nag. Raj and Kendrick)
and some growth characteristics in doubled haploid derivatives of the
F1 hybrid of tobacco (Nicotiana tabacum L.) - Anna
Trojak-Goluch and Apoloniusz Berbeć Abstract. Black root rot (BRR), caused by Chalara elegans, is a widespread and severe disease of tobacco (Nicotiana tabacum L.). Anther culture and stem culture techniques were used to produce the haploids and doubled haploids (DHR0) lines of F1 hybrids of N. tabacum cv. ‘K 326’ susceptible to BRR and cv. ‘Wentura’, a male parent of the widely cultivated BRR-resistant hybrid ‘VRG 2’. Twenty four mature DHR0 plants were self-pollinated and their derivatives (DHR1) were screened for BRR resistance in greenhouse tests. From a total of 24 DHR1 lines, seven (29.2%) had no necrotic symptoms while only fi ve of them (20.8%) were completely resistant. In a one-year study growth indicators and developmental characteristics of fi ve resistant DHR1 were compared to those of the parental cultivars in order to select BRR resistant lines with morphological traits of the reputable cv. ‘K326’. The DHR1 lines showed a considerable variation for basic growth parameters. Usually DHR1 fl owered later than cv. ‘K 326’ and ‘Wentura’. Three DHR1 lines had leaf number similar to cv. ‘Wentura’ while DHR128 line produced the number of leavesclose to that of cv. ‘K 326’. Most of the DHR1 lines produced the tallest plants compared to the parental cultivars. Signifi cant variation was observed among DHR1 for mid-stalk leaf parameters. Two lines DHR1 168 and DHR128 had leaves of length similar to that in ‘K 326’, however line DHR1 168 had wider leaves than cv. ‘K 326’. Finally one line DHR128 was selected as initial material for further breeding. key words: Nicotiana tabacum, Nicotiana debneyi, doubled haploids, black root rot, resistance |